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GAS3 Blocking Peptide CBP5101
  • Western blot analysis of GAS3 expression in THP1 (A), MDAMB435 (B), mouse brain (C), mouse liver (D) whole cell lysates.
  • Immunohistochemical analysis of GAS3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameGAS3 Blocking Peptide
Cat No: CBP5101
Source: Synthetic
Reactivity: H, M, R, B
Applications: BL
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
1 mg
$100
5 mg
$300
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Description: The peptide is used to block Anti-GAS3 Antibody (#CPA5101) reactivity.
Form: Lyophilized powder
Gene Symbol: PMP22
Alternative Names: GAS3; Peripheral myelin protein 22; PMP-22; Growth arrest-specific protein 3; GAS-3
Entrez Gene (Human): 5376
Entrez Gene (Mouse) : 18858
Entrez Gene (Rat) : 24660
SwissProt (Human): Q01453
SwissProt (Mouse) : P16646
SwissProt (Rat) : P25094
Purity : >85%
Directions for Use : Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours.
Quality Control : The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Storage/Stability : Shipped at 4°C. Store at -20°C for one year.
  • Western blot analysis of GAS3 expression in THP1 (A), MDAMB435 (B), mouse brain (C), mouse liver (D) whole cell lysates.
  • Immunohistochemical analysis of GAS3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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