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CEP135 Blocking Peptide CBP5075
  • Western blot analysis of CEP135 expression in HEK293T (A), mouse lung (B), mouse heart (C) whole cell lysates.
  • Immunohistochemical analysis of CEP135 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameCEP135 Blocking Peptide
Cat No: CBP5075
Source: Synthetic
Reactivity: H, M
Applications: BL
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
1 mg
$100
5 mg
$300
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Description: The peptide is used to block Anti-CEP135 Antibody (#CPA5075) reactivity.
Form: Lyophilized powder
Gene Symbol: CEP135
Alternative Names: CEP4; KIAA0635; Centrosomal protein of 135 kDa; Cep135; Centrosomal protein 4
Entrez Gene (Human): 9662
Entrez Gene (Mouse) : 381644
SwissProt (Human): Q66GS9
SwissProt (Mouse) : Q6P5D4
Purity : >85%
Directions for Use : Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours.
Quality Control : The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Storage/Stability : Shipped at 4°C. Store at -20°C for one year.
  • Western blot analysis of CEP135 expression in HEK293T (A), mouse lung (B), mouse heart (C) whole cell lysates.
  • Immunohistochemical analysis of CEP135 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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