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STAT5 Blocking Peptide CBP5061
  • Western blot analysis of STAT5 expression in HEK293T (A) whole cell lysates.
  • Immunohistochemical analysis of STAT5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameSTAT5 Blocking Peptide
Cat No: CBP5061
Source: Synthetic
Reactivity: H, M, R
Applications: BL
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
1 mg
$100
5 mg
$300
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Description: The peptide is used to block Anti-STAT5 Antibody (#CPA5061) reactivity.
Form: Lyophilized powder
Gene Symbol: STAT5A; STAT5B
Alternative Names: STAT5A; STAT5; Signal transducer and activator of transcription 5A; STAT5B; Signal transducer and activator of transcription 5B
Entrez Gene (Human): 6776; 6777
Entrez Gene (Mouse) : 20850; 20851
Entrez Gene (Rat) : 24918; 25126
SwissProt (Human): P42229; P51692
SwissProt (Mouse) : P42230; P42232
SwissProt (Rat) : Q62771; P52632
Purity : >85%
Directions for Use : Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours.
Quality Control : The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Storage/Stability : Shipped at 4°C. Store at -20°C for one year.
  • Western blot analysis of STAT5 expression in HEK293T (A) whole cell lysates.
  • Immunohistochemical analysis of STAT5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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