Description: The peptide is used to block Anti-p47 phox (pS345) Antibody (#CPA5058) reactivity. Form: Lyophilized powder Gene Symbol: NCF1 Alternative Names: NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phoxEntrez Gene (Human): 653361Entrez Gene (Mouse) : 17969SwissProt (Human): P14598SwissProt (Mouse) : Q09014Purity : >85%Directions for Use : Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours.Quality Control : The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.Storage/Stability : Shipped at 4°C. Store at -20°C for one year.

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  Western blot analysis of p47 phox (pS345) expression in HepG2 (A), NIH3T3 (B) whole cell lysates.
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  Immunohistochemical analysis of p47 phox (pS345) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.