Description: The peptide is used to block Anti-PPM1K Antibody (#CPA5021) reactivity. Form: Lyophilized powder Gene Symbol: PPM1K Alternative Names: PP2CM; Protein phosphatase 1K, mitochondrial; PP2C domain-containing protein phosphatase 1K; PP2C-like mitochondrial protein; PP2C-type mitochondrial phosphoprotein phosphatase; PTMP; Protein phosphatase 2C isoform kappa; PP2C-kappaEntrez Gene (Human): 152926Entrez Gene (Mouse) : 243382SwissProt (Human): Q8N3J5SwissProt (Mouse) : Q8BXN7Purity : >85%Directions for Use : Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours.Quality Control : The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.Storage/Stability : Shipped at 4°C. Store at -20°C for one year.
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Western blot analysis of PPM1K expression in HEK293T (A), human heart (B) whole cell lysates.
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Immunohistochemical analysis of PPM1K staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.