Description: The peptide is used to block Anti-CstF-64T Antibody (#CPA5004) reactivity. Form: Lyophilized powder Gene Symbol: CSTF2T Alternative Names: KIAA0689; Cleavage stimulation factor subunit 2 tau variant; CF-1 64 kDa subunit tau variant; Cleavage stimulation factor 64 kDa subunit tau variant; CSTF 64 kDa subunit tau variant; TauCstF-64Entrez Gene (Human): 23283Entrez Gene (Mouse) : 83410SwissProt (Human): Q9H0L4SwissProt (Mouse) : Q8C7E9Purity : >85%Directions for Use : Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours.Quality Control : The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.Storage/Stability : Shipped at 4°C. Store at -20°C for one year.
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Western blot analysis of CstF-64T expression in Hela (A), PC3 (B), MCF7 (C) whole cell lysates.
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Immunohistochemical analysis of CstF-64T staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.