Description: The peptide is used to block Anti-RPL5 Antibody (#CPA4996) reactivity. Form: Lyophilized powder Gene Symbol: RPL5 Alternative Names: 60S ribosomal protein L5Entrez Gene (Human): 6125Entrez Gene (Mouse) : 100503670Entrez Gene (Rat) : 81763SwissProt (Human): P46777SwissProt (Mouse) : P47962SwissProt (Rat) : P09895Purity : >85%Directions for Use : Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours.Quality Control : The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.Storage/Stability : Shipped at 4°C. Store at -20°C for one year.

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  Western blot analysis of RPL5 expression in Hela (A), Jurkat (B), MOLT4 (C) whole cell lysates.
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  Immunohistochemical analysis of RPL5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.