Description: The peptide is used to block Anti-PSMC3 Antibody (#CPA4989) reactivity. Form: Lyophilized powder Gene Symbol: PSMC3 Alternative Names: TBP1; 26S protease regulatory subunit 6A; 26S proteasome AAA-ATPase subunit RPT5; Proteasome 26S subunit ATPase 3; Proteasome subunit P50; Tat-binding protein 1; TBP-1Entrez Gene (Human): 5702Entrez Gene (Mouse) : 19182Entrez Gene (Rat) : 29677SwissProt (Human): P17980SwissProt (Mouse) : O88685SwissProt (Rat) : Q63569Purity : >85%Directions for Use : Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours.Quality Control : The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.Storage/Stability : Shipped at 4°C. Store at -20°C for one year.

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  Western blot analysis of PSMC3 expression in Hela (A), HepG2 (B), HT29 (C) whole cell lysates.
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  Immunohistochemical analysis of PSMC3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.