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CPA2389
  • Western blot analysis of Netrin 1 expression in Hela (A), U87MG (B), CT26 (C) whole cell lysates. (Predicted band size: 67 kD; Observed band size: 66 kD)
  • Immunohistochemical analysis of Netrin 1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-Netrin 1 Antibody
Cat No:CPA2389
Source:Rabbit
Reactivity:H, M, Mk
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to Netrin 1
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Netrin 1. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol:NTN1
Alternative Names:NTN1L; Netrin-1; Epididymis tissue protein Li 131P
Entrez Gene (Human): 9423;
Entrez Gene (Mouse): 18208;
SwissProt (Human): O95631;
SwissProt (Mouse): O09118;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Netrin 1 expression in Hela (A), U87MG (B), CT26 (C) whole cell lysates. (Predicted band size: 67 kD; Observed band size: 66 kD)
  • Immunohistochemical analysis of Netrin 1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Netrin-1 blockade inhibits tumour growth and EMT features in endometrial cancer
Journal Nature
IF 64.8
Application IHC
Reactivity Mouse
PMID 37532934
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