Description:Rabbit polyclonal antibody to Netrin 1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Netrin 1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:NTN1Alternative Names:NTN1L; Netrin-1; Epididymis tissue protein Li 131P
Entrez Gene (Human):
9423;
Entrez Gene (Mouse):
18208;
SwissProt (Human):
O95631;
SwissProt (Mouse):
O09118;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Netrin 1 expression in Hela (A), U87MG (B), CT26 (C) whole cell lysates. (Predicted band size: 67 kD; Observed band size: 66 kD)
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Immunohistochemical analysis of Netrin 1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Netrin-1 blockade inhibits tumour growth and EMT features in endometrial cancer