Description：Rabbit polyclonal antibody to PI3-kinase p150Immunogen：Recombinant fusion protein of human PI3-kinase p150. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol：PIK3R4Alternative Names：Phosphoinositide 3-kinase regulatory subunit 4; PI3-kinase regulatory subunit 4; PI3-kinase p150 subunit; Phosphoinositide 3-kinase adaptor protein Entrez Gene (Human)： 30849; Entrez Gene (Mouse)： 75669; Entrez Gene (Rat)： 363131; SwissProt (Human)： Q99570; SwissProt (Mouse)： Q8VD65; SwissProt (Rat)： P0C0R5; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PI3-kinase p150 expression in Raji (A), mouse brain (B) whole cell lysates. (Predicted band size: 153 kD; Observed band size: 150 kD)
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  Immunofluorescent analysis of PI3-kinase p150 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.