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CPA9295
  • Western blot analysis of GAPDH expression in Hela (A) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 37 kD)
  • Immunohistochemical analysis of GAPDH staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-GAPDH Antibody
Cat No:CPA9295
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
130
100 μl
80
30 μl
40
50 μl
55
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Description:Rabbit polyclonal antibody to GAPDH
Immunogen:Recombinant protein corresponding to human GAPDH.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 0.2% BSA, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/3000 - 1/10000), IH (1/50 - 1/100)
Gene Symbol:GAPDH
Alternative Names:GAPD; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
Entrez Gene (Human): 2597;
Entrez Gene (Mouse): 100042025;
Entrez Gene (Rat): 24383;
SwissProt (Human): P04406;
SwissProt (Mouse): P16858;
SwissProt (Rat): P04797;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of GAPDH expression in Hela (A) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 37 kD)
  • Immunohistochemical analysis of GAPDH staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Exosomal miRNA-155-5p from M1-polarized macrophages suppresses angiogenesis by targeting GDF6 to interrupt diabetic wound healing
Journal Mol Ther Nucleic Acids
IF 6.5
Application WB
Reactivity Human, Mouse
PMID 38074896
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