Description：Mouse monoclonal antibody to AMPK alpha 1Immunogen：KLH-conjugated synthetic peptide encompassing a sequence of human AMPK alpha 1. The exact sequence is proprietary.Purification：Affinity chromatographyClonality：MonoclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/1000 - 1/2000), IH (1/100 - 1/200)Gene Symbol：PRKAA1Alternative Names：AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1 Entrez Gene (Human)： 5562; SwissProt (Human)： Q13131; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of AMPK alpha 1 expression in Hela (A), 293T (B), NIH3T3 (C), PC12 (D) whole cell lysates. (Predicted band size: 64 kD; Observed band size: 64 kD)
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  Immunohistochemical analysis of AMPK alpha 1 staining in mouse colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.