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Bicinchoninic Acid Protein Assay Kit

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  • Source
  • Reactivity
  • Applications
    Protein quantification
Application key: E- ELISA, WB- Western blot, IH- Immunohistochemistry, IF- Immunofluorescence, FC- Flow cytometry, IC- Immunocytochemistry, IP- Immunoprecipitation, ChIP- Chromatin Immunoprecipitation, EMSA- Electrophoretic Mobility Shift Assay, BL- Blocking, SE- Sandwich ELISA, CBE- Cell-based ELISA, RNAi- RNA interference

Species reactivity key: H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish
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Description Bicinchoninic Acid Protein Assay Kit
Components Reagent A: 100 ml; stored at room temperature Reagent B: 2 ml; stored at room temperature Bovine Serum Albumin Standard: 100mg (Powder); stored at 4 C.
Application Protein quantification is often required before precessing protein samples for analysis. The Bicinchoninic Acid (BCA) Protein Assay is highly sensitive colorimetric assay that is not affected by chemicals in the sample. The BCA Protein Assay primarily reduces Cu2+ to Cu1+ by proteins in an alkaline environment followed by highly sensitive and selective colorimetric detection of BCA/copper complex. It is water-soluble and strongly absorbs light at 562nm in a linear fashion with increasing protein concentration.
Storage/Stability Store at 4 C.
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For research purposes only, not for human use
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