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hsa-miR-15a-5p miRNA Mimic

  • Cat No.
    CMH0007
  • Source
    Synthetic
  • Reactivity
    H
  • Applications
Application key: E- ELISA, WB- Western blot, IH- Immunohistochemistry, IF- Immunofluorescence, FC- Flow cytometry, IC- Immunocytochemistry, IP- Immunoprecipitation, ChIP- Chromatin Immunoprecipitation, EMSA- Electrophoretic Mobility Shift Assay, BL- Blocking, SE- Sandwich ELISA, CBE- Cell-based ELISA, RNAi- RNA interference

Species reactivity key: H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish
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Description Synthetic miRNA mimics are used to overexpress hsa-miR-15a-5p by transfaction.
Specificity miRNA Mimics are chemically modified; double-stranded miRNA-like RNA which are designed to copy the functionality of mature endogenous miRNA upon transfection. At the 5'-end; it is synthesized with a partially complementary motif to 3'UTR end of the target gene; which allows the miRNA mimic to specifically bind to the target. Transfection of mimics followed by downstream gene expression analysis or phenotypic analysis; is performed to elucidate the targets and roles of particular miRNAs.
Form Lyophilized powder
Gene Symbol hsa-miR-15a-5p
Entrez Gene (Human) MIMAT0000068
Directions for Use We recommend re-suspending the lyophilized synthetic miRNA using DNase and RNase-free ddH2O. To make a 100 uM stock; dissolve the lyophilized powder using 50 ul of ddH2O.
Components This synthetic miRNA is based on the mature miRNA sequence. It does not contain the full precursor miRNA stem-loop.
Storage/Stability Shipped at 4 deg C. Store at -20 deg C for one year. Avoid freeze-thaw cycles after reconstitution.
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For research purposes only, not for human use
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