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Line colors represent the approximate visible colors of the wavelength maxima.
Western blot analysis of Beta-actin expression in Jurkat (A), MCF7 (B), NIH3T3 (C), mouse brain (D), mouse liver (E), rat brain (F), rat liver (G) whole cell lysates. Antibody binding was detected using Goat Anti-Mouse IgG (H&L) - AcalephFluor790 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

Product Name:	Goat Anti-Mouse IgG (H&L) - AcalephFluor790
Cat No:	CSA4208
Source:	Goat
Reactivity:	M
Applications:	E, WB, IH, IF, FC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Size

Price(USD)

100 μl

500 μl

1 ml

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Datasheet

MSDS

Description：Goat Polyclonal Secondary Antibody to Mouse IgG (H&L) - AcalephFluor790

Immunogen：Mouse IgG

Purification：The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Clonality：Polyclonal

Conjugation：AcalephFluor790

Form：1 mg/ml. Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide

Dilution：WB (1/5000 - 1/20000), IH (1/100 - 1/1000), IF (1/100 - 1/1000), FC (1/1000 - 1/4000), E (Use at an assay dependent concentration)

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Platform：Ex/Em = 770/794 nm

Line colors represent the approximate visible colors of the wavelength maxima.
Western blot analysis of Beta-actin expression in Jurkat (A), MCF7 (B), NIH3T3 (C), mouse brain (D), mouse liver (E), rat brain (F), rat liver (G) whole cell lysates. Antibody binding was detected using Goat Anti-Mouse IgG (H&L) - AcalephFluor790 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

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