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CRP2981
Product Name:Recombinant Mouse IGF1 Protein
Cat No:CRP2981
Source:E. coli
Reactivity:M
Applications:E, WB, SDS-PAGE, MS
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
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Description:Recombinant Mouse IGF1 Protein is produced by E.coli expression system and the target gene encoding Gly49-Ala118 is expressed with a 6His tag at the C-terminus.
Form:Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 1mM EDTA, pH8.0.
Gene Symbol:IGF1
Alternative Names:IBP1; Insulin-like growth factor I; IGF-I; Mechano growth factor; MGF; Somatomedin-C
Entrez Gene (Mouse): 16000;
SwissProt (Mouse): P05017;
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Chemical Structure:MFPAMPLSSL FVNGGPETLC GAELVDALQF VCGPRGFYFN KPTGYGSSIR RAPQTGIVDE CCFRSCDLRR LEMYCAPLKP TKAALEHHHH HH
Directions for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Quality Control:Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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