Description:Recombinant Human SHH Protein is produced by E.coli expression system and the target gene encoding Cys24-Gly197 (Cys24Ile-Ile) is expressed.Form:Lyophilized from a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, 1mM DTT, pH 7.5.Gene Symbol:SHHAlternative Names:Sonic hedgehog protein; SHH; HHG-1
Entrez Gene (Human):
6469;
SwissProt (Human):
Q15465;
Purity:Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure:MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGGDirections for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.