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CRP2483
Product Name:Recombinant Human UBE2J2 Protein
Cat No:CRP2483
Source:E. coli
Reactivity:H
Applications:E, WB, SDS-PAGE, MS
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
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Description:Recombinant Human UBE2J2 Protein is produced by E. coli expression system and the target gene encoding Glu124-Arg224 is expressed with a GST tag at the N-terminus.
Form:Liquid in a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.2.
Gene Symbol:UBE2J2
Alternative Names:NCUBE2; Ubiquitin-conjugating enzyme E2 J2; Non-canonical ubiquitin-conjugating enzyme 2; NCUBE-2
Entrez Gene (Human): 118424;
SwissProt (Human): Q8N2K1;
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Chemical Structure:MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFH MEKGPTLGSI ETSDFTKRQL AVQSLAFNLK DKVFCELFPE VVEEIKQKQK AQDELSSRPQ TLPLPDVVPD GETHLVQNGI QLLNGHAPGA VPNLAGLQQA NR
Directions for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Storage/Stability:Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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