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CRP2482
Product Name:Recombinant Human UBE2I Protein
Cat No:CRP2482
Source:E. coli
Reactivity:H
Applications:E, WB, SDS-PAGE, MS
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
10 μg
60
50 μg
180
500 μg
1800
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Description:Recombinant Human UBE2I Protein is produced by E. coli expression system and the target gene encoding Met1-Ser158 is expressed with a GST tag at the N-terminus.
Form:Liquid in a 0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, pH 7.5.
Gene Symbol:UBE2I
Alternative Names:UBC9; UBCE9; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18
Entrez Gene (Human): 7329;
SwissProt (Human): P63279;
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Chemical Structure:MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMSG IALSRLAQER KAWRKDHPFG FVAVPTKNPD GTMNLMNWEC AIPGKKGTPW EGGLFKLRML FKDDYPSSPP KCKFEPPLFH PNVYPSGTVC LSILEEDKDW RPAITIKQIL LGIQELLNEP NIQDPAQAEA YTIYCQNRVE YEKRVRAQAK KFAPS
Directions for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Storage/Stability:Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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