Description:Recombinant Human UBE2C Protein is produced by E. coli expression system and the target gene encoding Met1-Pro179 is expressed with a 6His, T7 tag at the N-terminus.Form:Supplied as a 0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, pH 7.5.Gene Symbol:UBE2CAlternative Names:UBCH10; Ubiquitin-conjugating enzyme E2 C; UbcH10; Ubiquitin carrier protein C; Ubiquitin-protein ligase C
Entrez Gene (Human):
11065;
SwissProt (Human):
O00762;
Purity:Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSHMASQN RDPAATSVAA ARKGAEPSGG AARGPVGKRL QQELMTLMMS GDKGISAFPE SDNLFKWVGT IHGAAGTVYE DLRYKLSLEF PSGYPYNAPT VKFLTPCYHP NVDTQGNICL DILKEKWSAL YDVRTILLSI QSLLGEPNID SPLNTHAAEL WKNPTAFKKY LQETYSKQVT SQEPDirections for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.