Description:Recombinant Human TPP1 Protein is produced by Mammalian expression system and the target gene encoding Ser20-Pro563 is expressed with a 6His tag at the C-terminus.Form:Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM CaCl2, 10% Glycerol, pH 7.5.Gene Symbol:TPP1Alternative Names:CLN2; Tripeptidyl-peptidase 1; TPP-1; Cell growth-inhibiting gene 1 protein; Lysosomal pepstatin-insensitive protease; LPIC; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase I; TPP-I
Entrez Gene (Human):
1200;
SwissProt (Human):
O14773;
Purity:Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure:SYSPEPDQRR TLPPGWVSLG RADPEEELSL TFALRQQNVE RLSELVQAVS DPSSPQYGKY LTLENVADLV RPSPLTLHTV QKWLLAAGAQ KCHSVITQDF LTCWLSIRQA ELLLPGAEFH HYVGGPTETH VVRSPHPYQL PQALAPHVDF VGGLHRFPPT SSLRQRPEPQ VTGTVGLHLG VTPSVIRKRY NLTSQDVGSG TSNNSQACAQ FLEQYFHDSD LAQFMRLFGG NFAHQASVAR VVGQQGRGRA GIEASLDVQY LMSAGANIST WVYSSPGRHE GQEPFLQWLM LLSNESALPH VHTVSYGDDE DSLSSAYIQR VNTELMKAAA RGLTLLFASG DSGAGCWSVS GRHEFRPTFP ASSPYVTTVG GTSFQEPFLI TNEIVDYISG GGFSNVFPRP SYQEEAVTKF LSSSPHLPPS SYFNASGRAY PDVAALSDGY WVVSNRVPIP WVSGTSASTP VFGGILSLIN EHRILSGRPP LGFLNPRLYQ QHGAGLFDVT RGCHESCLDE EVEGQGFCSG PGWDPVTGWG TPNFPALLKT LLNPVDHHHH HHDirections for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability:Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.