Description:Recombinant Human TNF Protein is produced by E. coli. It is a single non-glycosylated polypeptide chain containing 151 amino acids with a molecular mass of 16886 Daltons.Form:Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.Gene Symbol:TNFAlternative Names:TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Entrez Gene (Human):
7124;
SwissProt (Human):
P01375;
Purity:Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.Directions for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.