Description:Recombinant Human SIT1 Protein is produced by E. coli expression system and the target gene encoding Gln65-Ser196 is expressed with a 6His tag at the N-terminus.Form:Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 250mM Nacl, 2mM EDTA, pH 8.0.Gene Symbol:SIT1Alternative Names:SIT; Signaling threshold-regulating transmembrane adapter 1; SHP2-interacting transmembrane adapter protein; Suppression-inducing transmembrane adapter 1; gp30/40
Entrez Gene (Human):
27240;
SwissProt (Human):
Q9Y3P8;
Purity:Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure:MGSSHHHHHH SSGLVPRGSH MQWTRGRSRS HPGQGRSGES VEEVPLYGNL HYLQTGRLSQ DPEPDQQDPT LGGPARAAEE VMCYTSLQLR PPQGRIPGPG TPVKYSEVVL DSEPKSQASG PEPELYASVC AQTRRARASF PDQAYANSQP AASLEHHHHH HDirections for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.