Description:Recombinant Human SHH Protein is produced by E. coli expression system and the target gene encoding Cys24-Gly197 is expressed.Form:Lyophilized from a 0.2 μM filtered solution of 20mM PB, 100mM NaCl, 1mM DTT, pH 7.5Gene Symbol:SHHAlternative Names:Sonic hedgehog protein; SHH; HHG-1
Entrez Gene (Human):
6469;
SwissProt (Human):
Q15465;
Purity:Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.Chemical Structure:MCGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKISRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGGDirections for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.