Description:Recombinant Human SCLY Protein is produced by E. coli expression system and the target gene encoding Met1-Ala445 is expressed with a 6His tag at the N-terminus.Form:Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.Gene Symbol:SCLYAlternative Names:SCL; Selenocysteine lyase; hSCL
Entrez Gene (Human):
51540;
SwissProt (Human):
Q96I15;
Purity:Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure:MGSSHHHHHH SSGLVPRGSH MEAAVAPGRD APAPAASQPS GCGKHNSPER KVYMDYNATT PLEPEVIQAM TKAMWEAWGN PSSPYSAGRK AKDIINAARE SLAKMIGGKP QDIIFTSGGT ESNNLVIHSV VKHFHANQTS KGHTGGHHSP VKGAKPHFIT SSVEHDSIRL PLEHLVEEQV AAVTFVPVSK VSGQTEVDDI LAAVRPTTRL VTIMLANNET GIVMPVPEIS QRIKALNQER VAAGLPPILV HTDAAQALGK QRVDVEDLGV DFLTIVGHKF YGPRIGALYI RGLGEFTPLY PMLFGGGQER NFRPGTENTP MIAGLGKAAE LVTQNCEAYE AHMRDVRDYL EERLEAEFGQ KRIHLNSQFP GTQRLPNTCN FSIRGPRLQG HVVLAQCRVL MASVGAACHS DHGDQPSPVL LSYGVPFDVA RNALRLSVGR STTRAEVDLV VQDLKQAVAQ LEDQADirections for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability:Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.