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CRP2225
Product Name:Recombinant Human RTN4 Protein
Cat No:CRP2225
Source:E. coli
Reactivity:H
Applications:E, WB, SDS-PAGE, MS
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
10 μg
130
50 μg
390
500 μg
2730
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Description:Recombinant Human RTN4 Protein is produced by E. coli expression system and the target gene encoding Met1-Val185 is expressed with a GST tag at the N-terminus.
Form:Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Gene Symbol:RTN4
Alternative Names:KIAA0886; NOGO; Reticulon-4; Foocen; Neurite outgrowth inhibitor; Nogo protein; Neuroendocrine-specific protein; NSP; Neuroendocrine-specific protein C homolog; RTN-x; Reticulon-5
Entrez Gene (Human): 57142;
SwissProt (Human): Q9NQC3;
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Chemical Structure:MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMEDL DQSPLVSSSD SPPRPQPAFK YQFVREPEDE EEEEEEEEED EDEDLEELEV LERKPAAGLS AAPVPTAPAA GAPLMDFGND FVPPAPRGPL PAAPPVAPER QPSWDPSPVS STVPAPSPLS AAAVSPSKLP EDDEPPARPP PPPPASVSPQ AEPVWTPPAP APAAPPSTPA APKRRGSSGS V
Directions for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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