Description：Recombinant Human RSPO3 Protein is produced by Mammalian expression system and the target gene encoding Gln22-His272 is expressed with a Fc, 6His tag at the C-terminus.Form：Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4.Gene Symbol：RSPO3Alternative Names：PWTSR; THSD2; R-spondin-3; Protein with TSP type-1 repeat; hPWTSR; Roof plate-specific spondin-3; hRspo3; Thrombospondin type-1 domain-containing protein 2 Entrez Gene (Human)： 84870; SwissProt (Human)： Q9BXY4; Purity：Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.Chemical Structure：QNASRGRRQR RMHPNVSQGC QGGCATCSDY NGCLSCKPRL FFALERIGMK QIGVCLSSCP SGYYGTRYPD INKCTKCKAD CDTCFNKNFC TKCKSGFYLH LGKCLDNCPE GLEANNHTME CVSIVHCEVS EWNPWSPCTK KGKTCGFKRG TETRVREIIQ HPSAKGNLCP PTNETRKCTV QRKKCQKGER GKKGRERKRK KPNKGESKEA IPDSKSLESS KEIPEQRENK QQQKKRKVQD KQKSVSVSTV HVDDIEGRMD EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHHDirections for Use：Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control：Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability：Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.