Description:Recombinant Human NEDD8 Protein is produced by E. coli expression system and the target gene encoding Met1-Gly76 is expressed with a 6His, SUMO tag at the N-terminus.Form:Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, pH 7.4.Gene Symbol:NEDD8Alternative Names:NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8
Entrez Gene (Human):
4738;
SwissProt (Human):
Q15843;
Purity:Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure:MGHHHHHHGS LQDSEVNQEA KPEVKPEVKP ETHINLKVSD GSSEIFFKIK KTTPLRRLME AFAKRQGKEM DSLRFLYDGI RIQADQAPED LDMEDNDIIE AHREQIGGML IKVKTLTGKE IEIDIEPTDK VERIKERVEE KEGIPPQQQR LIYSGKQMND EKTAADYKIL GGSVLHLVLA LRGGDirections for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.