Description:Recombinant Human MGMT Protein is produced by E. coli expression system and the target gene encoding Met1-Asn207 is expressed with a 6His tag at the N-terminus.Form:Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 1mM EDTA, pH 8.0.Gene Symbol:MGMTAlternative Names:Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase
Entrez Gene (Human):
4255;
SwissProt (Human):
P16455;
Purity:Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure:MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPALHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRNDirections for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability:Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.