Description:Recombinant E. coli MAP Protein is produced by E.coli expression system and the target gene encoding Ala2-Glu264 is expressed with a 6His tag at the C-terminus.Form:Supplied as a 0.2 μM filtered solution of 20mM Tris-HCl, 100mM NaCl, 50% Glycerol, pH 8.0.Gene Symbol:MAPAlternative Names:Methionine aminopeptidase; MAP; MetAP; Peptidase M
Entrez Gene (Human):
947882 (E. coli);
SwissProt (Human):
P0AE18 (E. coli);
Purity:Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.Chemical Structure:AISIKTPEDI EKMRVAGRLA AEVLEMIEPY VKPGVSTGEL DRICNDYIVN EQHAVSACLG YHGYPKSVCI SINEVVCHGI PDDAKLLKDG DIVNIDVTVI KDGFHGDTSK MFIVGKPTIM GERLCRITQE SLYLALRMVK PGINLREIGA AIQKFVEAEG FSVVREYCGH GIGRGFHEEP QVLHYDSRET NVVLKPGMTF TIEPMVNAGK KEIRTMKDGW TVKTKDRSLS AQYEHTIVVT DNGCEILTLR KDDTIPAIIS HDELEHHHHH HDirections for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.