Description:Recombinant Human MAN1B1 Protein is produced by Mammalian expression system and the target gene encoding Asp106-Ala699 is expressed with a 6His tag at the C-terminus.Form:Liquid in a 0.2 μM filtered solution of 50mM Tris-HCI, 10mM reduced Glutathione, pH 8.0.Gene Symbol:MAN1B1Alternative Names:Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase; ER alpha-1,2-mannosidase; ER mannosidase 1; ERMan1; Man9GlcNAc2-specific-processing alpha-mannosidase; Mannosidase alpha class 1B member 1
Entrez Gene (Human):
11253;
SwissProt (Human):
Q9UKM7;
Purity:Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.Chemical Structure:DHWKALAFRL EEEQKMRPEI AGLKPANPPV LPAPQKADTD PENLPEISSQ KTQRHIQRGP PHLQIRPPSQ DLKDGTQEEA TKRQEAPVDP RPEGDPQRTV ISWRGAVIEP EQGTELPSRR AEVPTKPPLP PARTQGTPVH LNYRQKGVID VFLHAWKGYR KFAWGHDELK PVSRSFSEWF GLGLTLIDAL DTMWILGLRK EFEEARKWVS KKLHFEKDVD VNLFESTIRI LGGLLSAYHL SGDSLFLRKA EDFGNRLMPA FRTPSKIPYS DVNIGTGVAH PPRWTSDSTV AEVTSIQLEF RELSRLTGDK KFQEAVEKVT QHIHGLSGKK DGLVPMFINT HSGLFTHLGV FTLGARADSY YEYLLKQWIQ GGKQETQLLE DYVEAIEGVR THLLRHSEPS KLTFVGELAH GRFSAKMDHL VCFLPGTLAL GVYHGLPASH MELAQELMET CYQMNRQMET GLSPEIVHFN LYPQPGRRDV EVKPADRHNL LRPETVESLF YLYRVTGDRK YQDWGWEILQ SFSRFTRVPS GGYSSINNVQ DPQKPEPRDK MESFFLGETL KYLFLLFSDD PNLLSLDAYV FNTEAHPLPI WTPAVDHHHH HHDirections for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability:Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.