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CRP1668
Product Name:Recombinant Human HMBS Protein
Cat No:CRP1668
Source:Human cells
Reactivity:H
Applications:E, WB, SDS-PAGE, MS
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
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Description:Recombinant Human HMBS Protein is produced by mammalian expression system and the target gene encoding Ser2-His361 is expressed with a 6His tag at the C-terminus.
Form:Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Gene Symbol:HMBS
Alternative Names:PBGD; UPS; Porphobilinogen deaminase; PBG-D; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase
Entrez Gene (Human): 3145;
SwissProt (Human): P08397;
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Chemical Structure:SGNGNAAATA EENSPKMRVI RVGTRKSQLA RIQTDSVVAT LKASYPGLQF EIIAMSTTGD KILDTALSKI GEKSLFTKEL EHALEKNEVD LVVHSLKDLP TVLPPGFTIG AICKRENPHD AVVFHPKFVG KTLETLPEKS VVGTSSLRRA AQLQRKFPHL EFRSIRGNLN TRLRKLDEQQ EFSAIILATA GLQRMGWHNR VGQILHPEEC MYAVGQGALG VEVRAKDQDI LDLVGVLHDP ETLLRCIAER AFLRHLEGGC SVPVAVHTAM KDGQLYLTGG VWSLDGSDSI QETMQATIHV PAQHEDGPED DPQLVGITAR NIPRGPQLAA QNLGISLANL LLSKGAKNIL DVARQLNDAH VDHHHHHH
Directions for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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