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CRP1653
Product Name:Recombinant Human HAO1 Protein
Cat No:CRP1653
Source:E. coli
Reactivity:H
Applications:E, WB, SDS-PAGE, MS
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
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Description:Recombinant Human HAO1 Protein is produced by E. coli expression system and the target gene encoding Met1-Ile370 is expressed with a Trx, 6His tag at the N-terminus.
Form:Liquid in a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Gene Symbol:HAO1
Alternative Names:GOX1; HAOX1; Hydroxyacid oxidase 1; HAOX1; Glycolate oxidase; GOX
Entrez Gene (Human): 54363;
SwissProt (Human): Q9UJM8;
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Chemical Structure:MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAG SGSGHMHHHH HHSSGLVPRG SGMKETAAAK FERQHMDSPD LGTDDDDKAM ADIGSMLPRL ICINDYEQHA KSVLPKSIYD YYRSGANDEE TLADNIAAFS RWKLYPRMLR NVAETDLSTS VLGQRVSMPI CVGATAMQRM AHVDGELATV RACQSLGTGM MLSSWATSSI EEVAEAGPEA LRWLQLYIYK DREVTKKLVR QAEKMGYKAI FVTVDTPYLG NRLDDVRNRF KLPPQLRMKN FETSTLSFSP EENFGDDSGL AAYVAKAIDP SISWEDIKWL RRLTSLPIVA KGILRGDDAR EAVKHGLNGI LVSNHGARQL DGVPATIDVL PEIVEAVEGK VEVFLDGGVR KGTDVLKALA LGAKAVFVGR PIVWGLAFQG EKGVQDVLEI LKEEFRLAMA LSGCQNVKVI DKTLVRKNPL AVSKI
Directions for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Storage/Stability:Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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