Description:Recombinant Human ALPP Protein is produced by Mammalian expression system and the target gene encoding Ile23-Asp506 is expressed with a 6His tag at the C-terminus.Form:Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 0.2mM GaCl, 10% Glycerol, pH 7.5Gene Symbol:ALPPAlternative Names:PLAP; Alkaline phosphatase, placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1
Entrez Gene (Human):
250;
SwissProt (Human):
P05187;
Purity:Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.Chemical Structure:IIPVEEENPD FWNREAAEAL GAAKKLQPAQ TAAKNLIIFL GDGMGVSTVT AARILKGQKK DKLGPEIPLA MDRFPYVALS KTYNVDKHVP DSGATATAYL CGVKGNFQTI GLSAAARFNQ CNTTRGNEVI SVMNRAKKAG KSVGVVTTTR VQHASPAGTY AHTVNRNWYS DADVPASARQ EGCQDIATQL ISNMDIDVIL GGGRKYMFRM GTPDPEYPDD YSQGGTRLDG KNLVQEWLAK RQGARYVWNR TELMQASLDP SVTHLMGLFE PGDMKYEIHR DSTLDPSLME MTEAALRLLS RNPRGFFLFV EGGRIDHGHH ESRAYRALTE TIMFDDAIER AGQLTSEEDT LSLVTADHSH VFSFGGYPLR GSSIFGLAPG KARDRKAYTV LLYGNGPGYV LKDGARPDVT ESESGSPEYR QQSAVPLDEE THAGEDVAVF ARGPQAHLVH GVQEQTFIAH VMAFAACLEP YTACDLAPPA GTTDVDHHHH HHDirections for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability:Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.