Description:TMB ELISA Substrate detects horseradish peroxidase (HRP) activity.Form:Liquid
Directions for Use:(a) After incubation with horseradish peroxidase (HRP) conjugated secondary antibody, wash plates with PBS/0.05% Tween-20.
(b) Add 100 μl of TMB solution/well. Incubate for a specific amount of time or until desired signal is achieved.
(c) Add 100 μl of ELISA Stop Solution.
(d) Read at Absorbance 450 nm.Components:TMB is a one component system that is ready to use from the bottle.Storage/Stability:Store at 4 °C in dark for 1 year, do not freeze.