Description：Rabbit polyclonal antibody to NOB1Immunogen：Recombinant fusion protein of human NOB1. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：NOB1Alternative Names：ART4; NOB1P; PSMD8BP1; RNA-binding protein NOB1; Phosphorylation regulatory protein HP-10; Protein ART-4 Entrez Gene (Human)： 28987; Entrez Gene (Mouse)： 67619; Entrez Gene (Rat)： 291996; SwissProt (Human)： Q9ULX3; SwissProt (Mouse)： Q8BW10; SwissProt (Rat)： Q6VEU1; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NOB1 expression in LO2 (A), rat testis (B) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 47 kD)
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  Immunofluorescent analysis of NOB1 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).