Description：Rabbit polyclonal antibody to ITSN2Immunogen：Recombinant fusion protein of human ITSN2. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：ITSN2Alternative Names：KIAA1256; SH3D1B; SWAP; Intersectin-2; SH3 domain-containing protein 1B; SH3P18; SH3P18-like WASP-associated protein Entrez Gene (Human)： 50618; SwissProt (Human)： Q9NZM3; SwissProt (Mouse)： Q9Z0R6; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ITSN2 expression in Jurkat (A), HeLa (B) whole cell lysates. (Predicted band size: 135; 141; 190; 193 kD; Observed band size: 180 kD)
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  Immunofluorescent analysis of ITSN2 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).