Description：Rabbit polyclonal antibody to ITCHImmunogen：KLH-conjugated synthetic peptide of human ITCH. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol：ITCHAlternative Names：E3 ubiquitin-protein ligase Itchy homolog; Itch; Atrophin-1-interacting protein 4; AIP4; NFE2-associated polypeptide 1; NAPP1 Entrez Gene (Human)： 83737; Entrez Gene (Mouse)： 16396; SwissProt (Human)： Q96J02; SwissProt (Mouse)： Q8C863; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ITCH expression in HEK293T (A) whole cell lysates. (Predicted band size: 103 kD; Observed band size: 103 kD)
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  Immunohistochemical analysis of ITCH staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.