Description：Rabbit polyclonal antibody to HSP70Immunogen：KLH-conjugated synthetic peptide of human HSP70. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200), IP (1/50 - 1/200)Gene Symbol：HSPA1A; HSPA1BAlternative Names：HSPA1; HSX70; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2 Entrez Gene (Human)： 3303; 3304; Entrez Gene (Rat)： 24472; 294254; SwissProt (Human)： P0DMV8; P0DMV9; SwissProt (Rat)： Q07439; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HSP70 expression in HeLa (A), NIH3T3 (B), HepG2 (C) whole cell lysates. (Predicted band size: 70 kD; Observed band size: 70 kD)
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  Immunofluorescent analysis of HSP70 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.