Description:Rabbit polyclonal antibody to STCHImmunogen:Recombinant fusion protein of human STCH. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:HSPA13Alternative Names:STCH; Heat shock 70 kDa protein 13; Microsomal stress-70 protein ATPase core; Stress-70 protein chaperone microsome-associated 60 kDa protein
Entrez Gene (Human):
6782;
Entrez Gene (Mouse):
110920;
Entrez Gene (Rat):
29734;
SwissProt (Human):
P48723;
SwissProt (Mouse):
Q8BM72;
SwissProt (Rat):
O35162;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of STCH expression in U87MG (A) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 65 kD) -
Immunofluorescent analysis of STCH staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.


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