Description：Rabbit polyclonal antibody to hnRNP DImmunogen：Recombinant fusion protein of human hnRNP D. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol：HNRNPDLAlternative Names：HNRPDL; JKTBP; Heterogeneous nuclear ribonucleoprotein D-like; hnRNP D-like; hnRNP DL; AU-rich element RNA-binding factor; JKT41-binding protein; Protein laAUF1 Entrez Gene (Human)： 9987; Entrez Gene (Rat)： 305178; SwissProt (Human)： O14979; SwissProt (Mouse)： Q9Z130; SwissProt (Rat)： Q3SWU3; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of hnRNP D expression in SW620 (A), mouse testis (B), rat brain (C) whole cell lysates. (Predicted band size: 27; 33; 46 kD; Observed band size: 38 kD)
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  Immunofluorescent analysis of hnRNP D staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.