Description：Rabbit polyclonal antibody to HEY1Immunogen：KLH-conjugated synthetic peptide of human HEY1. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：HEY1Alternative Names：BHLHB31; CHF2; HERP2; HESR1; HRT1; Hairy/enhancer-of-split related with YRPW motif protein 1; Cardiovascular helix-loop-helix factor 2; CHF-2; Class B basic helix-loop-helix protein 31; bHLHb31; HES-related repressor protein 1; Hairy and enhancer of split-related protein 1; HESR-1; Hairy-related transcription factor 1; HRT-1; hHRT1 Entrez Gene (Human)： 23462; SwissProt (Human)： Q9Y5J3; SwissProt (Mouse)： Q9WV93; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HEY1 expression in HEK293T (A), mouse heart (B) whole cell lysates. (Predicted band size: 32; 33 kD; Observed band size: 33 kD)
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  Immunofluorescent analysis of HEY1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).