Description:Rabbit polyclonal antibody to ARL13BImmunogen:Recombinant fusion protein of human ARL13B. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:ARL13BAlternative Names:ARL2L1; ADP-ribosylation factor-like protein 13B; ADP-ribosylation factor-like protein 2-like 1; ARL2-like protein 1
Entrez Gene (Human):
200894;
Entrez Gene (Mouse):
68146;
SwissProt (Human):
Q3SXY8;
SwissProt (Mouse):
Q640N2;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of ARL13B expression in SKOV3 (A) whole cell lysates. (Predicted band size: 36; 37; 48 kD; Observed band size: 49 kD)
-
Immunofluorescent analysis of ARL13B staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).