Description:Rabbit polyclonal antibody to APOBEC3FImmunogen:Recombinant fusion protein of human APOBEC3F. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol:APOBEC3FAlternative Names:DNA dC->dU-editing enzyme APOBEC-3F; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F; A3F
Entrez Gene (Human):
200316;
SwissProt (Human):
Q8IUX4;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of APOBEC3F expression in Hela (A), HepG2 (B) whole cell lysates. (Predicted band size: 9; 11; 45 kD; Observed band size: 45 kD)
-
Immunohistochemical analysis of APOBEC3F staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.