Description：Rabbit polyclonal antibody to ANO2Immunogen：Recombinant fusion protein of human ANO2. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol：ANO2Alternative Names：C12orf3; TMEM16B; Anoctamin-2; Transmembrane protein 16B Entrez Gene (Human)： 57101; Entrez Gene (Mouse)： 243634; SwissProt (Human)： Q9NQ90; SwissProt (Mouse)： Q8CFW1; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ANO2 expression in mouse brain (A) whole cell lysates. (Predicted band size: 113 kD; Observed band size: 114 kD)
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  Immunofluorescent analysis of ANO2 staining in Y79 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.