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CQA3811
  • Western blot analysis of ANKLE2 expression in Jurkat (A) whole cell lysates. (Predicted band size: 104 kD; Observed band size: 104 kD)
  • Immunohistochemical analysis of ANKLE2 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-ANKLE2 Antibody
Cat No:CQA3811
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
50 μl
150
30 μl
110
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Description:Rabbit polyclonal antibody to ANKLE2
Immunogen:Recombinant fusion protein of human ANKLE2. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)
Gene Symbol:ANKLE2
Alternative Names:KIAA0692; LEM4; Ankyrin repeat and LEM domain-containing protein 2; LEM domain-containing protein 4
Entrez Gene (Human): 23141;
Entrez Gene (Mouse): 71782;
Entrez Gene (Rat): 360829;
SwissProt (Human): Q86XL3;
SwissProt (Mouse): Q6P1H6;
SwissProt (Rat): Q7TP65;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of ANKLE2 expression in Jurkat (A) whole cell lysates. (Predicted band size: 104 kD; Observed band size: 104 kD)
  • Immunohistochemical analysis of ANKLE2 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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