Datasheet
MSDSDescription：Rabbit polyclonal antibody to NUMBImmunogen：Recombinant full length protein of human NUMBPurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：NUMBAlternative Names：Protein numb homolog; h-Numb; Protein S171
Entrez Gene (Human)：
8650;
Entrez Gene (Mouse)：
18222;
SwissProt (Human)：
P49757;
SwissProt (Mouse)：
Q9QZS3;
SwissProt (Rat)：
Q2LC84;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NUMB expression in Hela (A), A549 (B), rat liver (C) whole cell lysates. (Predicted band size: 37; 48-70 kD; Observed band size: 71 kD) -
Immunofluorescent analysis of NUMB staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).