Description：Rabbit polyclonal antibody to Septin 9Immunogen：Recombinant full length protein of human Septin 9Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：SEPT9Alternative Names：KIAA0991; MSF; Septin-9; MLL septin-like fusion protein MSF-A; MLL septin-like fusion protein; Ovarian/Breast septin; Ov/Br septin; Septin D1 Entrez Gene (Human)： 10801; Entrez Gene (Mouse)： 53860; Entrez Gene (Rat)： 83788; SwissProt (Human)： Q9UHD8; SwissProt (Mouse)： Q80UG5; SwissProt (Rat)： Q9QZR6; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Septin 9 expression in Jurkat (A), Hela (B) whole cell lysates. (Predicted band size: 38; 41; 47; 52; 63; 64; 65 kD; Observed band size: 75 kD)
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  Immunofluorescent analysis of Septin 9 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).