Description：Rabbit polyclonal antibody to CHCHD3Immunogen：Recombinant full length protein of human CHCHD3Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IP (1/10 - 1/50)Gene Symbol：CHCHD3Alternative Names：MINOS3; Coiled-coil-helix-coiled-coil-helix domain-containing protein 3 mitochondrial Entrez Gene (Human)： 54927; Entrez Gene (Mouse)： 66075; SwissProt (Human)： Q9NX63; SwissProt (Mouse)： Q9CRB9; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of CHCHD3 expression in A431 (A), HepG2 (B), mouse heart (C) whole cell lysates. (Predicted band size: 26 kD; Observed band size: 30 kD)
• 
  Immunofluorescent analysis of CHCHD3 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).