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CQA3487
  • Western blot analysis of SMEK1 expression in Jurkat (A), MCF7 (B), mouse spleen (C), rat brain (D) whole cell lysates. (Predicted band size: 62; 67; 93; 95 kD; Observed band size: 110 kD)
  • Immunohistochemical analysis of SMEK1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-SMEK1 Antibody
Cat No:CQA3487
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
50 μl
150
30 μl
110
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Description:Rabbit polyclonal antibody to SMEK1
Immunogen:Recombinant full length protein of human SMEK1
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Gene Symbol:SMEK1
Alternative Names:KIAA2010; PP4R3A; PPP4R3A; Serine/threonine-protein phosphatase 4 regulatory subunit 3A; SMEK homolog 1
Entrez Gene (Human): 55671;
Entrez Gene (Mouse): 68734;
SwissProt (Human): Q6IN85;
SwissProt (Mouse): Q6P2K6;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of SMEK1 expression in Jurkat (A), MCF7 (B), mouse spleen (C), rat brain (D) whole cell lysates. (Predicted band size: 62; 67; 93; 95 kD; Observed band size: 110 kD)
  • Immunohistochemical analysis of SMEK1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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